Identification of Major and Minor Allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus)
- Author:
Syuhaidah Sahabudin
;
Rosmilah Misnan
;
Zailatul Hani Mohammad Yadzir
;
Jamaludin Mohamad
;
Noormalin Abdullah
;
Faizal Bakhtiar
;
Shahnaz Murad
- Publication Type:Journal Article
- Keywords:
allergens, allergy and clinical immunology, hypersensitivity, Penaeus, immunoblotting, tropomyosin
- From:Malaysian Journal of Medical Sciences
2011;18(3):27-32
- CountryMalaysia
- Language:English
-
Abstract:
Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE
antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and
king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of
thi s study was to identify the IgE-binding proteins of these 2 prawn species.
Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium
dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then
performed using sera from patients with positive skin prick tests to the raw prawn extracts.
Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein
bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were
sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts
of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with
molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the
major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen
in black tiger prawns. Other potential allergens were also observed at various molecular masses.
Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both
species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine
kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major
allergen in black tiger prawns.
- Full text:W020151022583092060399.pdf
