Spasmogenic Activity of the Seed of Terminalia chebula Retz in Rat Small Intestine: In Vivo and In Vitro Studies
- Author:
Seyyed Ali Mard
;
Ali Veisi
;
Mohammad Kazem Gharib Naseri
;
Peyman Mikaili
- Publication Type:Journal Article
- Keywords:
calcium channels, gut, intestinal motility, morphine, plant extracts, rat, seed, Terminalia
- From:Malaysian Journal of Medical Sciences
2011;18(3):18-26
- CountryMalaysia
- Language:English
-
Abstract:
Background: Terminalia chebula Retz is traditionally used to relieve constipation. The
current study was performed to investigate the pharmacological action of aqueous extract of
Terminalia chebula seeds (ATC) in vitro and in vivo.
Methods: Terminal pieces of rat ileum were suspended in organ bath containing Tyrode
solution. The ileum spontaneous motility frequency and contractility were recorded isotonically.
To induce ileal contraction, carbachol and ATC were added to the organ bath. In addition, the effect
of hexamethonium, indomethacin, atropine, and verapamil on the ATC-induced ileal contractions
was also investigated. The effectiveness of ATC on relieving morphine-induced constipation was
investigated in an in vivo study by measuring the faecal number, faecal water content, and intestinal
transit ratio.
Results: ATC increased the frequency of ileum motility and tension of contraction dosedependently
(P < 0.05). Responses induced by ATC were inhibited by pre-treatment of the tissue with
verapamil. The ATC activities were not affected by atropine, hexamethonium, and indomethacin.
The faecal number and faecal water content were increased dose-dependently by ATC (P < 0.05).
Conclusion: The excitatory effects of ATC on ileal contractile frequency and tension are
possibly mediated through Ca2+ channels activation. The results of the present study support the
traditional usage of ATC for the treatment of constipation.
- Full text:W020151022582582694413.pdf
