Antiproliferative Properties of Clausine-B against Cancer Cell Lines
- Author:
Wan Nor I’zzah Wan Mohd Zain
;
Asmah Rahmat
;
Fauziah Othman
;
Taufiq Yun Hin Yap
- Publication Type:Journal Article
- Keywords:
Clausine-B, Clausena excavata, ethnopharmacology, cell survival, medical sciences
- From:Malaysian Journal of Medical Sciences
2009;16(3):31-36
- CountryMalaysia
- Language:English
-
Abstract:
Background: Clausine B, a carbazole alkaloid isolated from the stem bark of Clausena excavata,
was investigated for its antiproliferative activities against five human cancer cell lines: HepG2 (hepatic
cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast
cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer).
Methods: Chang liver (normal cells) was used as a control. The effect of clausine-B was measured
using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.
Results: Clausine-B was found to be active (IC50<30 μg/mL) against four of the cancer cell lines
tested. The IC50 values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa),
27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at
52.90 μg/mL, and no IC50 value was obtained against Chang liver.
Conclusion: It is possible that the phenolic group in clausine-B responsible for the antiproliferative
activities found in this study.
- Full text:W020151022412723472558.pdf
