In vitro cytotoxicity evaluation of dental porcelain using human cell lines
- Author:
Hazem Yousef Abu Sharbeh
;
Thirumulu Ponnuraj Kannana
;
Raja Azman Raja Awang
;
Adam Husein
- Publication Type:Journal Article
- Keywords:
Dental Porcelain, cell line, cytotoxicity, MTT assay
- From:Archives of Orofacial Sciences
2012;7(2):68-74
- CountryMalaysia
- Language:English
-
Abstract:
The in vitro cytotoxic potential of locally produced dental porcelain was evaluated in this study. The cellular response of human osteoblast and fibroblast cell lines were assessed using MTT assay by incubating with the fluid extract of dental porcelain powder and dental porcelain discs (direct test). Aging process was carried out by submerging the discs into 3% bovine serum albumin (BSA) solution. Tests on extracts showed that dental porcelain was significantly different from the control at a concentration of 250 mg/ml. Direct test showed that dental porcelain after aging was not significantly different from the control with a mean (SD) of 89.2 (13.4)%, whereas, it was significantly different from the control before conditioning of BSA with a mean (SD) of 88.5 (12.1)%. However, the dental porcelain caused mild suppression of succinate dehydrogenase activity (<25%), which is considered to be accepted clinically and hence can be ranked as being non-cytotoxic.
- Full text:W020150629399681015869.pdf
