Genotoxicity assessment of locally produced dental nanocomposite using Comet assay
- Author:
Siti Robayah Mohd Zakri
;
Thirumulu Ponnuraj Kannan *
;
Nora Aziz
;
Siti Fadilah Abdullah
;
Dasmawati Mohamad
;
Ismail Ab Rahman
;
Abdul Rashid Ismail
- Publication Type:Journal Article
- Keywords:
Comet assay, DNA damage, nanocomposite, SHED.
- From:Archives of Orofacial Sciences
2011;6(1):15-20
- CountryMalaysia
- Language:English
-
Abstract:
The aim of this study was to determine the
genotoxicity of a locally produced nanocomposite by Universiti
Sains Malaysia, Malaysia using Comet assay. Stem cells from
human exfoliated deciduous teeth (SHED) were treated with
the nanocomposite at five different concentrations (0.006,
0.0125, 0.025, 0.05, and 0.1 mg/ml) along with concurrent
negative (medium alone) and positive control (zinc sulfate
heptahydrate) and incubated at 37°C for 24 hours in an
incubator at 5% CO2. The tail moment was used to assess the
extent of DNA damage. The tail moment for the group of SHED
treated with nanocomposite (for all the five different
concentrations) was not statistically significant as compared to
the negative control, suggesting that the locally produced
dental nanocomposite did not induce any DNA damage.
Hence, it can be concluded that the locally produced
nanocomposite is non-genotoxic on stem cells from human
exfoliated deciduous teeth.
- Full text:W020150629335398201012.pdf
