The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn
- Author:
Carolina Sisca Djunaidi
;
Francis Maria Constance Sigit Setyabudi
;
Sardjono
- Publication Type:Journal Article
- Keywords:
Aflatoxin B1;
Detoxification;
Solid Substrate;
Aspergillus oryzae KKB4
- From:Malaysian Journal of Microbiology
2017;13(2):117-123
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The growth and metabolic activity of Aspergillus oryzae KKB4 in AFB1-contaminated corn and later coincided
with AFB1 reduction and detoxification were investigated.
Methodology and results: The decreasing of AFB1 amount by A. oryzae KKB4 could be clearly observed if the initial
AFB1 concentration in corn was increased. Thus, moist-heated corn was artificially inoculated with Aspergillus flavus
FNCC 62C7 to increase AFB1 content. AFB1-contaminated corn was applied as solid substrate and then inoculated with
A. oryzae KKB4. During fermentation periods, the growth, metabolic activity, and AFB1 decline were investigated by
glucosamine content, water content loss, and AFB1 concentration, respectively. The maximum growth was occurred in
4
thday at 1.499 ± 0.028 g glucosamine/ 100 g dry matter. The metabolic activity was going on up to the end of
fermentation days, as shown as water content loss at 0.175 ± 0.007 g/g dry matter. In accordance with the growth and
metabolic activity, the amount of AFB1 reduction was 37.04 ng AFB1/g dry matter during 5 days fermentation. According
to toxicity analysis, it was found that the residues of AFB1 were not toxic to Bacillus megaterium cells.
Conclusion, significance and impact of study: A. oryzae KKB4 is able to be applied in solid substrates as AFB1
reduction and detoxification agent. These lucrative effects are also important in relation with food and feed safety.
- Full text:P020170616443399676697.pdf
