Investigation of phylogroups and some virulence traits among cervico-vaginal Escherichia coli (CVEC) isolated for female in Hilla City, Iraq
- Author:
Marwa Mohammed Al-Khaqani
;
Mourouge Saadi Alwash
;
Hussein Oleiwi Al-Dahmoshi
- Publication Type:Journal Article
- Keywords:
CVEC;
phylogrouping;
chuA;
yjaA;
biofilm
- MeSH:
Vaginosis, Bacterial
- From:Malaysian Journal of Microbiology
2017;13(2):132-138
- CountryMalaysia
- Language:English
-
Abstract:
Aims: This study aims to investigate the phylogroups, antibiotics susceptibility and biofilm formation among CVEC
isolated from female with bacterial vaginosis.
Methodology and results: High vaginal swab from girl with age (18-60 years) were collected and cultured on
MacConkey agar, EMB agar and UTI chromogenic medium to recover CVEC and only the confirmed Escherichia coli will
pass through rest of the assays like phylogrouping (by PCR), antibiotics susceptibility test and biofilm formation. The
results revealed that only 32 (20.38%) of CVEC were recovered and among them only 3 (9.375%) of CVEC belong to
intestinal subgroup A1 and the rest 29 (90.625%) assigned to extraintestinal phylogenetic group B2. CVEC isolates
belong to B1 and D groups not reported. Antibiotics resistance results shown that, 32 (100%) for cefazolin, cephalothin,
cefoxitin and metronidazole, 31 (96.9%) for erythromycin, 24 (75%) for fosfomycin, 20 (62.5%) for cefotaxime, 16 (50%)
for ceftazidime, 14 (43.75%) for cefepeim, (28.1%) for aztreonam, 7 (21.9%) for streptomycin, 6 (18.75%) for
meropenem, 5 (15.6%) for both imipenem and gentamicin, 2 (6.25%) for both ciprofloxacin and norfloxacin, amikacin 1
(3.1%) and no resistance stated for nitrofurantion (0.00%). TCP methods results display that 12 (37.5%) of CVEC were
biofilm former while 20 (62.5%) were non biofilm former.
Conclusion, significance and impact of study: This study concluded that, most of the CVEC belong to highly virulent
phylogroup B2 and have the ability to resist multiple antibiotics and the ciprofloxacin, norfloxacin, amikacin and
nitrofurantion still the best choice for treatment and CVEC have the ability to form biofilm which make the infection
ruthless and hard to cure.
- Full text:P020170616430747630455.pdf
