Identification and lactic acid production of bacteria isolated from soils and tree barks
- Author:
Vasana Tolieng
;
Budsabathip Prasirtsak
;
Jaruwan Sitdhipol
;
Nuttha Thongchul
;
Somboon Tanasupawat
- Publication Type:Journal Article
- Keywords:
Bacillus;
Enterococcus;
Lactococcus;
Sporolactobacillus;
lactic acid
- MeSH:
Lactic Acid
- From:Malaysian Journal of Microbiology
2017;13(2):100-108
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The objective of this research was to isolate and identify lactic acid producing bacteria from soils and tree barks in
Thailand. Their acid production was also determined.
Methodology and results: Eleven bacterial strains isolated from soils and tree barks were screened for their lactic acid
production. They were divided into 4 groups based on their phenotypic characteristics and 16S rRNA gene sequence
analyses. Group I (3 isolates) identified as Lactococcus produced L-lactic acid ranged from 72.32 ± 0.707-77.47 ± 0.184
g/L, yield of 0.96 ± 0.011-1.06 ± 0.008 g/g, productivity of 1.00 ± 0.010-1.08 ± 0.003 g/L.h and optical purity was 100%.
Group II (3 isolates) identified as Enterococcus hirae, produced L-lactic acid ranged from 31.56 ± 0.424-34.86 ± 0.283
g/L, yield of 1.06 ± 0.008-1.23 ± 0.036 g/g, productivity of 0.44 ± 0.006-0.48 ± 0.004 g/L.h and optical purity was 98.6-
100%. Group III (3 isolates) identified as Bacillus coagulans, produced L-lactic acid ranged from 48.48 ± 0.283-93.51 ±
0.552 g/L, yield of 1.00 ± 0.001-1.07 ± 0.005 g/g, productivity of 0.67 ± 0.004-1.30 ± 0.008 g/L.h and high optical purity of
the tested isolate was 99.56%. Group IV (2 isolates) identified as Sporoactobacillus. Only NN2 produced D-lactic acid
concentration 87.64 ± 0.375 g/L, yield of 0.83 ± 0.000 g/g, productivity of 1.22 ± 0.005 g/L.h and optical purity was
96.16%. All isolates produced lactic acid when 120 g/L of the initial glucose was used as a substrate.
Conclusion, significance and impact of study: Lactic acid producing bacteria are isolated from soils and tree barks.
The coccal isolates could produce high L-lactic acid concentration with high optical purity while the spore-forming
isolates produces L- and D-lactic acid which are useful for food preservation, chemical in pharmaceutical, cosmetic and
textile industries.
- Full text:P020170616427091658636.pdf
