Effect of biofertilizer on the diversity of nitrogen - fixing bacteria and total bacterial community in lowland paddy fields in Sukabumi West Java, Indonesia
- Author:
Masrukhin
;
Iman Rusmana
;
Nisa Rachmania Mubarik
- Publication Type:Journal Article
- Keywords:
Biofertilizer;
Diazotroph;
nifH;
metagenomic;
DGGE
- From:Malaysian Journal of Microbiology
2017;13(2):139-146
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Some of methanotrophic bacteria and nitrous oxide (N2O) reducing bacteria have been proven able to support the
plant growth and increase productivity of paddy. However, the effect of application of the methanotrophics and N2O
reducing bacteria as a biofertilizer to indigenous nitrogen-fixing bacteria and total bacterial community are still not well
known yet. The aim of the study was to analyze the diversity of nitrogen-fixing bacteria and total bacterial communty in
lowland paddy soils.
Methodology and results: Soil samples were taken from lowland paddy fields in Pelabuhan Ratu, Sukabumi, West
Java, Indonesia. There were two treatments applied to the paddy field i.e biofertilizer-treated field (biofertilizer with 50
kg/ha NPK) and control (250 kg/ha NPK fertilizer). There were nine different nifH bands which were successfully
sequenced and most of them were identified as unculturable bacteria and three of them were closely related to
Sphingomonas sp., Magnetospirillum sp. and Ideonella dechloratans respectively. In addition, there were 20 different
16S rDNA bands which were successfully sequenced. Phylogenetic analysis of the sequence showed that there were 5
phyla of bacteria, i.e. Proteobacteria (Alphaproteobacteria and Gammaproteobacteria), Chlorofexi, Gemmatimonadetes,
Clostridia, and Bacteroidetes respectively. Alphaproteobacteria was the most dominant group in lowland paddy field.
Microbial diversities in the biofertilizer-treated field were lower than that of 100% fertilizer-treated field either based on
nifH and 16S rDNA genes.
Conclusion, significance and impact study: Biofertilizer treatment has lower microbial diversity than control, either
based on nifH and 16S rDNA genes.
- Full text:P020170616417815431342.pdf
