Streptomycetes associated with marine sponges collected from Andaman Sea and its antibacterial activity
- Author:
Ram Baskaran
;
Subramanian Thenmozhi
;
P.M. Mohan
- Publication Type:Original Article
- Keywords:
Marine sponges, Streptomyces, diversity, bioactivity, Andaman Islands
- From:Malaysian Journal of Microbiology
2017;13(3):253-260
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Sponges harbour diverse prokaryotic and eukaryotic microbes. However, diversity of sponge-derived
Actinomycetes is of high interest because of its bioactive compounds. In the present study, diversity of Streptomycetes
associated with marine sponges collected from Pongibalu (south Andaman) was investigated.
Methodology and results: Sponges samples were collected by underwater SCUBA diver and Kuster’s agar media was
used for isolation of actinobacteria. Colony morphology and 16s rRNA were studied for identification of isolates and
phylogenetic tree was constructed using MEGA.6. A total of ten Streptomyces species were identified based on 16S
rRNA gene sequencing from three sponge species (Hemiasterella spp. Tentorium spp. and Tethyopsis spp.). The
organic extracts of these ten isolates revealed bioactivity against tow Gram positive and eight Gram negative pathogenic
bacteria.
Conclusion, significance and impact of study: This study suggests prospects and potentials of the diverse population
of Streptomyces with bioactivity in marine sponges. It would be a potential source in the pharmaceutical industries. As
well as actinobacteria associated with sponge may prevent the sponge from pathogenic bacteria, fungi, virus and other
microflora by secretion of secondary metabolites on surface and inside. To understand the sponge and actinobacteria
association and its bioactivity, a profound study need to be conducted.