Ability of endophytic fungi isolated from Nepenthes ampullaria to degrade polyurethane
- Author:
Shirley Bong Wuan Lii
;
Changi Wong
;
Muller
- Publication Type:Original Article
- Keywords:
Biodegradation, Pestalotiopsis, Nepenthes, polyurethane, proteome
- From:Malaysian Journal of Microbiology
2017;13(3):172-179
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Waste electric and electronic equipment (WEEE) are among the fastest growing waste products worldwide and
solutions to their remediation are urgently needed. Bioremediation is a green approach that is helpful to minimize
environmental pollution associated with Electronic waste (E-waste). The present study aimed at exploring the potential
of endophytic fungi from Nepenthes ampullaria for bioremediation purposes of the plastic component in E-waste,
polyurethane (PUR) polymers.
Methodology and results: Endophytic fungal isolates were assessed for their ability to degrade PUR as well as their
ability to utilise PUR as sole carbon source. Nine (9) out of 150 isolates demonstrated the ability to efficiently degrade
polyurethane in solid medium and the top three (3) isolates were able to grow on PUR as the only carbon source. These
three isolates were identified using ITS1 and ITS4 and found to be closely related to the genus Pestalotiopsis. The top
two of the three isolates were then assessed for their esterase enzyme activity as well as changes in their proteome
when grown with and without PUR. The highest enzymatic activity was found to be 1850.4 U/mL when tested using pnitrophenol
acetate as the substrate. Analyses of the 2-dimensional electrophoresis profile revealed changes in the
abundance of proteins when treated with polyurethane.
Conclusion, significance and impact of study: This study is to our knowledge the first on endophytes isolated from N.
ampullaria that can degrade PUR, and also their proteomes. Results obtained from this study can in the future help to
reduce polyurethane wastes. Besides degrading PUR polymer, endophytic fungi produce potential valuable proteins that
may find broad applications in bioremediation applications.
