Identification and characterization of lactic acid bacteria isolated from fruit tree rhizosphere in MARDI, Malaysia
- Author:
Nurul Ain Abu Bakar
;
Mohammad Hariz Abdul Rahman
;
Nur Alyani Shakri
- Publication Type:Journal Article
- Keywords:
morphology;
soil LAB;
antibiotic susceptibility;
16S rRNA;
16S rDNA
- MeSH:
Lactic Acid
- From:Malaysian Journal of Microbiology
2017;13(1):xx-xx
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Lactic acid bacteria (LAB) have promising applications in the biotechnology industry. As their diversity in soil is
largely unexplored, a study was undertaken to collect LAB from the soil, and to characterize the isolates.
Methodology and results: Soil samples from around various fruit trees in MARDI were collected for LAB isolation by
accumulation/incubation method. The isolates were examined for their morphological and biochemical characteristics,
and their 16S rDNA sequences. Morphological and biochemical analysis showed that all isolates are gram positive with
different characteristic for each isolates. Further identification were performed and soil samples was found to contain
diverse genera and species of LAB, including Lactobacillus sp. and Bacillus sp. Selected isolates were tested for
resistance against six antibiotics using agar dilution method. Widespread antibiotic resistance among the strains tested
was found towards ampicillin, kanamycin, rifampicin and penicillin.
Conclusion, significance and impact study: LAB have the ability in producing acid and antimicrobial compound which
are useful in various industry. Fastidious characteristics of LAB are among the limitations and challenges in industrial
applications. Diversity of lactic acid-producing bacteria was encountered in the soil which may be useful and have
different characteristics from LAB isolates elsewhere.
- Full text:P020170308382301636295.pdf
