First report of Erwinia psidii associated with papaya dieback disease in Malaysia
- Author:
Wan Ting Chai
;
Jualang Azlan Gansau
;
Markus Atong
;
Jugah Kadir
;
Evenni Poili
;
Khim Phin Chong
- Publication Type:Journal Article
- Keywords:
Papaya dieback disease;
Koch’s Postulates;
Erwinia psidii;
Sabah;
16S rRNA
- From:Malaysian Journal of Microbiology
2017;13(1):xx-xx
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The outbreak of papaya dieback disease in Malaysia has been reported since 2003. Several reports previously
confirmed Erwinia papayae and E. mallotivora to be the causal pathogen of the disease. The present study aimed to
identify the causal pathogen of papaya dieback disease in Sabah.
Methodology and results: Infected tissues of papaya dieback disease were collected from Kota Belud, Sabah and the
bacterium responsible for the infection was isolated on Luria Bertani (LB) agar and nutrient agar (NA). Seven isolates
with similar characteristics to Erwinia were isolated, subjected to the Koch’s Postulates test and then identified using 16S
rRNA sequencing technique. The bacterium was identified to be E. psidii, a common pathogen to guava but not to
papaya.
Conclusion, significance and Impact of study: This report serves as the first confirmation of the E. psidii in causing
papaya dieback disease, suggesting the possibility of this bacterium undergoing host shifting to papaya plants and the
possibility of becoming another major threat to the papaya industry in the future.
- Full text:P020170308361129918081.pdf
