Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)
- Author:
Shey-Li Lim
;
Huda Mohd Nor Nabila
;
Subki Atiqah
;
Sreeramanan Subramaniam
;
Lai-Keng Chan
;
Ishak Zamzuri
;
Hamzah Ghazali Amir
- Publication Type:Journal Article
- Keywords:
beneficial interaction;
embryogenic calli;
Herbaspirillum seropedicae Z78;
in vitro oil palm
- From:Malaysian Journal of Microbiology
2016;12(6):485-491
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The purpose of this experiment was to determine the artificial symbiosis interaction of Herbaspirillum seropedicae
(Z78) on oil palm embryogenic calli.
Methodology and results: For this purpose, symbiotic associations were established between Z78 and embryogenic
calli of oil palm tissue cultured. A total of five treatments involved, in particular: i) + 3.0 mg/L 2,4-D + 100% N MS
medium (control), ii) + Z78 pellet cells (1 mL) + 25% N MS medium, iii) + Z78 supernatant (1 mL) + 25% N MS medium,
iv) + Z78 broth culture (1 mL) + 25% N MS medium, and v) + Z78 sonicated cells (1 mL) + 25% N MS medium. All
treatments were supplied with minimal N sources (25% N), ammonium nitrate and potassium nitrate, while the control
was treated with 100% N sources. Treated samples were harvested on D80 and observed for biomass and diameter
increment (%), formation of embryoids, and Z78 colonization. The results showed embryogenic calli in the inoculated
treatments that contained depleted N produced similar result to the control treatment which contained 100% N nutrients.
Positive interactions occurred between the diazotroph and host plant tissues as viewed under FESEM and EFTEM.
Among the treatments, Z78 sonicated cell showed better growth of embryogenic calli compared to others.
Conclusion, significance and impact study: The in vitro nitrogen-depleted artificial symbiosis environment allowed
the diazotroph (Z78) to be expressed and provide the nitrogen sources and indole-3-acetic acid for cell growth. This
study represents beneficial co-culture interaction effects of different inocula of diazotrophic bacterial cells with in vitro
embryogenic calli of oil palm.
- Full text:P020170203372352387075.pdf
