Antimicrobial, total phenolic and total flavonoid properties of leaves and seed of Jatropha curcas, Piper nigrum L. and Piper betle methanolic crude extracts
- Author:
Mohamad Iswandy Ibrahim
;
Awang Ahmad Sallehin Awang Husaini
;
Noorzaid Muhamad
;
Hairul Azman Roslan
- Publication Type:Journal Article
- Keywords:
antimicrobial;
phenolic;
flavonoid;
phytochemical screening;
Piper betle
- MeSH:
Therapeutics
- From:Malaysian Journal of Microbiology
2016;12(6):438-444
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Herbal and medicinal plants bioactive compounds of Jatropha curcas, Piper nigrum and P. betle have been
shown to possess therapeutic properties. This study investigates the presence and characterization of phytochemical
compounds as well as to evaluate the antimicrobial activities of the methanolic crude extract of the leaves and seeds of
J. curcas, P. nigrum L. and P. betle.
Methodology and results: The study on antibacterial and antifungal efficacy of the crude extracts of leaves and seeds
were carried out using standard disc diffusion method. The crude extracts were found to exhibit an average response of
antimicrobial activity with the inhibition zones ranged from 3% to 28% for antibacterial and from 21% to 79% for
antifungal activity. Among all extracts, the leaf extract of P. betle showed a good antibacterial activity against
Staphylococcus aureus and excellent antifungal properties against Aspergillus niger and A. flavus. The phytochemical
screening analysis revealed the presence of saponin, tannins, glycosides, terpenoids, reducing sugar, flavonoid and
anthraquinones. However, phlobatannins was not present. Total phenolic content (TPC) and total flavonoid content
(TFC) were highly detected in the crude extract of P. betle and recorded as 13.33 mg of gallic acid equivalents, GAE
(mg/ 100 mg sample) and 0.88 mg of RE (mg/100 mg sample), respectively. GC-MS analysis of the bioactive
compounds reveals the presence of diethyl phthalate, 2-hexadecen-1-ol (Phytol), hexadecanoic acid, piperine, phenol
and other minor compounds.
Conclusion, significance and impact of study: The study suggested that P. betle has a potential as a source for
antimicrobial agent from plants extracts. Nevertheless, further studies are needed to elucidate their precise mechanism
of action.
- Full text:P020170203335883479514.pdf
