Outer membrane proteins and lipopolysaccharides mediated antibody response against bovine Pasteurella multocida type B-2
- Author:
Imran Altaf
;
Anika Khalid
;
Jawad Nazir
;
Waseem Shahzad
;
Rasheeda Bashir
- Publication Type:Journal Article
- Keywords:
Complement fixation test;
lipopolysaccharides;
outer membrane proteins;
Pasteurella multocida
- From:Malaysian Journal of Microbiology
2016;12(5):370-375
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The activation of cellular and humoral immunity depends upon nature of antigens. Complex proteins like bacterial
outer membrane proteins (OMP) usually successfully activate both humoral and cellular immunity. Whereas antigens
like bacterial lipopolysaccharides (LPS) usually elicit T-independent immunity i.e. humoral immunity without the
activation of cellular immune wing. The present study was under taken to evaluate the comparative immunologic
behavior of both the important molecules (bacterial lipopolysaccharide and outer membrane proteins) of Pasteurella
multocida alone and in combination in bovine calves in field conditions.
Methodology and results: Pasteurella multocida was isolated, purified and identified from an outbreak by mean of
culture and biochemical methods. The pathogenicity of the confirmed isolates was done in rabbits (Oryctolagus
cuniculus) on the principles of Koch’s postulates. Alum based vaccine against P. multocida was prepared and antibody
titer against Outer membrane protein (OMP) and lipopolysaccharides (LPS) were determined by complement fixation
test (CFT). The results showed that the antibody titer against OMP and LPS in whole culture vaccine is significantly
higher than the respective tested vaccines. These results concluded that OMP no doubt is an active T-dependent
immunogenic molecule but its immunogenicity increases many times when combined with LPS in whole culture vaccine.
Conclusion, significance and impact of study: Lipopolysaccharides (LPS) in combination with outer membrane
proteins (OMP) synergistically boost the humoral immune response in vaccinated animal.
- Full text:P020170106344315113613.pdf
