Phytochemical, antimicrobial and mast cell stabilizing activity of ethanolic extract of Solanum trilobatum Linn. leaves
- Author:
Subramani Parasuraman
;
Lee Yu Ren
;
Bobby Lau Chik Chuon
;
Stephanie Wong Kah Yee
;
Tan Ser Qi
;
Jan Yong Shu Ching
;
Parayil Varghese Christapher
;
Krishnamoorthy Venkateskumar
;
Palanimuthu Vasanth Raj
- Publication Type:Journal Article
- Keywords:
antimicrobial;
GC-MS analysis;
mast cell stabilizing activit;
, Solanum trilobatum
- From:Malaysian Journal of Microbiology
2016;12(5):359-364
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Solanum trilobatum Linn., (Solanaceae) is one of the most widely used plants as food supplement in southern
part of India and some parts of Southeast Asia. This plant is traditionally used for the treatment of respiratory illness. In
animal studies, the extract of S. trilobatum showed significant antimicrobial, hepatoprotective and anticancer activities.
The complete phytochemical profile, antimicrobial and mast cell stabilizing activities of S. trilobatum remains unclear.
This study tests the antimicrobial, antihistaminic and mast cell stabilizing activities of ethanolic extract of leaves of S.
trilobatum (EEST).
Methodology and results: The phytochemical test was carried out using chemical and instrumental [Gas
Chromatography Mass Spectrometry (GC-MS)] analytical methods. Antimicrobial effect of EEST was tested against
Streptococcus pneumonia, Escherichia coli and Staphylococcus aureus. Intestinal mesentery of Sprague Dawley (SD)
rats was used to study the peritoneal mast cell stabilization activity of EEST. The rat intestinal mesentery was exposed
to 50, 100, 200, 300, 400 and 600 μg/mL of EEST and the peritoneal mast cell stabilization activity was compared with
that of standards (pheniramine 20 μg/mL and ketotifen 20 μg/mL). The phytochemical test showed the presence of
carbohydrates, saponins, flavonoids, alkaloids, tannins and phenolic compounds. GC-MS analysis indicated the
presence of 45 fragmented compounds which included epoxylinalol, himachalol, illudol, epibuphanamine, baimuxinal
and edulan IV. EEST exhibited antimicrobial activity at 10 mg/mL against S. aureus, S. pneumonia. Significant mast cell
stabilizing activity was observed from the dose of 100 μg/mL to 600 μg/mL.
Conclusion: Ethanolic extract of leaves of S. trilobatum possess significant antimicrobial and antihistaminic activity.
- Full text:P020170106331572300270.pdf
