Antimicrobial effects of biosynthesized silver nanoparticles produced by Actinomyces spp. based on their sizes and shapes
- Author:
Negar Andisheh
;
Majid Baserisalehi
- Publication Type:Journal Article
- Keywords:
Silver nanoparticle;
Actinomyces;
antimicrobial effect;
pathogenic microorganisms
- MeSH:
Actinomyces
- From:Malaysian Journal of Microbiology
2016;12(4):327-331
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Actinomyces are dominant soil microflora with potent activity for production of several enzymes and metabolites.
In order to increase their survival in the environment these bacteria detoxify the metal ions and consequently produce
the nanoparticles. The present study was undertaken to isolate Actinomyces strains from soil samples and their
evaluation for the production of silver nanoparticles with antimicrobial property.
Methodology and results: Two hundred soil samples were collected and subjected to isolation and identification
(based on16SrRNA gene sequencing) of silver nanoparticles producing Actinomyces. The silver nanoparticles produced
by Actinomyces were confirmed by UV-visible spectral analysis, scanning electron microscope (SEM) and Inductively
Coupled Plasma (ICP). Furthermore, antimicrobial property of silver nanoparticles was assessed against pathogenic
microorganisms viz., Staphylococcus aureus (PTCC 1431), Acinetobacter baumannii (PTCC 19606), Bacillus cereus
(PTCC 1816), Escherichia coli (PTCC 1397), Salmonella typhi (PTCC 1609), Pseudomonas aeruginosa (PTCC 1707),
Aspergillus niger (PTCC 5010) and Candida albicans (PTCC 5072). Of 48 Actinomyces isolated, 26 strains could
produce silver nanoparticles and three of which showed potent activity for production of silver nanoparticles. Molecular
identification of these strains exhibited detection of Actinomyces amycolicicoccus subflavus, Streptomyces flavoviridis
and Streptomyces lateritius. The results obtained from characterization of the biosynthesis silver nanoparticles illustrated
that their shapes and sizes were spindle and spherical and 47-103 nm respectively. However, the antimicrobial effect of
silver nanoparticles against the pathogenic microorganisms was varied. Yet S. typhi followed by P. aeruginosa, were
more sensitive and A. baumannii was relatively less sensitive. In addition, spherical shape with small average size
relatively showed more antimicrobial property.
Conclusion, significance and impact of study: Soil Actinomyces could produce silver nanoparticles and these
particles have antimicrobial effect. In addition, the antimicrobial effect of silver nanoparticles, not only because of their
chemical property (such as formation of free radical) but also depended on their shapes and sizes.
- Full text:P020170105555812146559.pdf
