The role of gene polymorphisms of glutamate-cysteine ligase catalytic (GCLC) enzyme against antioxidants and oxidative stress status of Individual who had contacted infectious tuberculosis
- Author:
Muh Nasrum Massi1
;
Sitti Rafiah
;
Rusdina Bte Ladju
;
Gaby Maulida Nurdin
;
Andi Zulkifli
;
Ari Yuniastuti
- Publication Type:Journal Article
- Keywords:
pulmonary TB contacts;
GCLC gene;
glutathione levels;
F2-isoprostanes
- MeSH:
Glutamate-Cysteine Ligase
- From:Malaysian Journal of Microbiology
2016;12(4):322-326
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Glutamate cysteine ligase (GCL) enzyme is involved in the synthesis of glutathione, which functions as an
antioxidant. Polymorphisms in the sequence of amino acids making up the gene GCLC will cause differences in enzyme
expression and GCLC activity. Gene expression that is influenced by oxidative stress can be used to measure markers
such as F2-isoprostanes. This study aims to examine the association between the polymorphism in the GCLC gene with
glutathione plasma level and F2-isoprostanes in contacts of person with infectious tuberculosis (TB).
Methodology and results: Samples are taken from the family members of pulmonary TB patients who seeks treatment
at the Pulmonary Centre (Lung Health Center for Public = BBKPM) and Policlinic of Dr Wahidin Sudirohusodo Hospital,
Makassar. Total of approximately 4 mL of venous blood are taken from each person with pulmonary TB contacts and
furtherly analyzed using genomic PCR-RFLP method and ELISA. Our results described that contacts of person with
infectious TB for approximately 6 months have polymorphism C/C genotype at 80.3%, C/T of 18.3% and T/T for 1.4% of
the total 71 samples with high levels of glutathione from 0.167 to 0.548 mM/mL and F2-isoprostanes level 72.4 - 1343.9
pg/mL.
Conclusion, significance and impact of study: There are no significant association between GCLC gene
polymorphism with glutathione and F2-isoprostanes levels of individual who had contacted infection TB. In this study the
elevation of F2-isoprostanes equal to the decrease levels of glutathione.
- Full text:P020170105552291056536.pdf
