Influence of pH, temperature and nutrient addition on the degradation of atrazine by Nocardioides spp. isolated from agricultural soil in Nigeria
- Author:
Ayodele Elizabeth Omotayo
;
Matthew Olusoji Ilori
;
Oluwafemi Sunday Obayori
;
Olukayode Oladipo Amund
- Publication Type:Journal Article
- Keywords:
atrazine;
degradation;
pH;
temperature;
Nocardioides
- MeSH:
Atrazine
- From:Malaysian Journal of Microbiology
2016;12(4):270-278
- CountryMalaysia
- Language:English
-
Abstract:
Aims: To effectively exploit the atrazine degrading capabilities of Nocardioides spp. isolated from agricultural soil
samples in Nigeria and ascertain the effect of pH, temperature and nutrient addition on the degradation process.
Methodology and results: Isolates were cultivated on atrazine mineral salts medium at a temperature range of 4 °C -
45 °C and a pH range of 3-10. An optimum atrazine degrading activity was observed in the isolates between
temperatures of 25 °C and 37 °C and between pH 5 and 8. Different carbon sources (glycerine, glucose, chitin, cellulose
and sodium citrate) and nitrogen sources (urea, biuret, cyanuric acid, potassium nitrate and ammonium chloride) were
also added to the medium. The addition of carbon and nitrogen sources did not increase degradation rates although
urea and glycerine repressed the degradation ability of the isolates. Statistical analyses of variance at P < 0.05 showed
no significant differences in the growth and degradation rates by both bacterial isolates under these conditions.
Conclusion, significance and impact study: Atrazine degradation by Nocardioides spp. is pH and temperature
dependent, and requires no additional sources of carbon and nitrogen. Hence, its use in bioremediation of atrazine
contaminated agricultural soil should be explored.
- Full text:P020170105499851995910.pdf
