Screening of bacteria for PHA production using waste glycerol as carbon source and the ability of new strain to produce P(3HB-co-3HV) copolymer
- Author:
Khansaa Badie Al-Kaddo
;
Kumar Sudesh
;
Mohd Razip Samian
- Publication Type:Journal Article
- Keywords:
Burkholderia contaminans;
waste glycerol;
sodium valerate;
P(3HB-co-3HV) copolymer
- MeSH:
bacteria
- From:Malaysian Journal of Microbiology
2016;12(3):245-253
- CountryMalaysia
- Language:English
-
Abstract:
Aims: This study is focused on the isolation, characterization and screening of new bacterial strains isolated from soil
and wastewater samples that are able to produce PHA utilizing waste glycerol as sole carbon source in order to create
useful products from waste glycerol and at the same time reduce the PHA production cost. A new isolate, Burkholderia
contaminans Kad1 strain was investigated for its ability to biosynthesize PHA copolymers containing 3HV monomers
from waste glycerol and 3-hydroxyvalerate (3HV) precursors.
Methodology and results: PHA producing bacteria were screened using Nile Red and 1% of Nile Blue method. The
presence of PHA granules was detected using phase contrast and fluorescence microscopy. Burkholderia contaminans
Kad1, one out of 23 positive samples, was selected for further study because of its ability to produce high PHA content
(47 wt%) and dry cell weight (DCW), (4.2 g/L) when waste glycerol 2% (v/v) was used as the sole carbon source. The
16S rDNA and the PHA synthase gene were sequenced and the PHA produced was confirmed by NMR analysis. A
mixture of waste glycerol and sodium valerate fed to the culture gave rise to poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) [P(3HB-co-3HV)]. The mole fraction of 3HV monomer in the co-polymer P(3HB-co-3HV) sample
analyzed using 1H NMR was 23 mol%.
Conclusion, significance and impact of study: This study demonstrated for the first time B. contaminans Kad1 was
able to use waste glycerol for PHA biosynthesis including the P(3HB-co-3HV) copolymer using a mixture of waste
glycerol with sodium valerate as the precursor.
- Full text:P020170105441313705321.pdf
