In vitro antiviral activity of styrylpyrone derivative-incorporated formulations against Herpes simplex virus type-1
- Author:
Adibah Ahamad Bahtiar
;
Norefrina Shafinaz Md Nor
;
Nazlina Ibrahim
- Publication Type:Journal Article
- Keywords:
HSV-1;
antiviral;
Goniothalamus umbrosus;
styrylpyrone derivative
- MeSH:
Herpesvirus 1, Human
- From:Malaysian Journal of Microbiology
2016;12(3):228-232
- CountryMalaysia
- Language:English
-
Abstract:
Aims: This study was aimed to evaluate in vitro antiviral activity of topical formulations incorporated with a styrylpyrone
derivative (SPD) against Herpes Simplex Virus type 1 (HSV-1).
Methodology and results: Two types of SPD-incorporated formulations (ointment and gel) were tested for their antiviral
activity against HSV-1 clinical strain using plaque reduction assay on Vero cells. The antiviral activity was determined
based on the percentage of plaque reduction occurred between treatment and control (non-treated infected cells). In this
study, 10% SPD-gel (SPD = 0.004 mg) and 20% SPD-ointment (SPD = 0.003 mg) showed plaque reduction percentage
of 87% and 79% respectively. Further evaluation on the ointment base, gel base (formulation without SPD)
demonstrated less than 10% of antiviral activity while pure SPD at 0.0025 mg showed 81% of plaque reduction. These
results indicated that the antiviral activity observed in both SPD-incorporated ointment and gel was mainly due to SPD
regardless of formulation components. Furthermore, the antiviral activities observed in both SPD-incorporated products
were also in agreement with the antiviral activity observed in pure SPD.
Conclusion, significance and impact study: SPD-incorporated products retained the antiviral activity and can further
be tested in animal model.
- Full text:P020170105419995114048.pdf
