Culturable and unculturable actinomycetes associated with the sponge Neofibularia from Bira Island, Indonesia
- Author:
Cico Jhon Karunia Simamora
;
Dedy Duryadi Solihin
;
Yulin Lestar
- Publication Type:Journal Article
- Keywords:
Actinomycetes;
culturable;
DGG;
, Neofibularia sp.;
unculturable
- MeSH:
Actinobacteria
- From:Malaysian Journal of Microbiology
2016;12(3):211-220
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The diversity of the actinomycete community associated with Neofibularia sp. from Bira Island, Indonesia, has
been largely unstudied. This study was undertaken to address the paucity of information in this respect.
Methodology and results: Culturable actinomycetes were isolated and cultured on HV medium. Polymerase chain
reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the metagenomic 16S rRNA was used to
analyse the structure of the actinomycete community. Five culturable actinomycetes that were isolated belonged to the
genus Streptomyces. They showed various degrees of similarity to the reference strains Streptomyces sampsonii (97-
99%), Streptomyces resistomycificus (97-99%), Streptomyces gougerotii (97-99%), Streptomyces erringtonii (97-99%),
and Streptomyces albus (97-99%). The culturable actinomycetes isolates also showed differences in morphological
characteristics as compared with the reference strains. The metagenomic analysis suggested that the actinomycete
community was dominated by rare actinomycetes. Eight DGGE DNA bands that were obtained had sequences that
showed similarities to Ferrithrix thermotolerans (88-94%), Lamia majanohamensis (87-92%), Aciditerrimonas
ferrireducens (87-92%), and Thermobispora bispora (85-92%), while 4 bands had sequences similar to
Propionibacterium acnes (97-100%) and another band matched sequences belonging to an uncultured bacterium clone
(86-87%). The actinomycetes detected by the metagenomic approach were assigned identities that were mostly under
97.5% as compared with reference strains available in Genbank.
Conclusion, significance and impact of study: Observations from both culture and DGGE analysis give a better
understanding of the diversity and community structure of actinomycetes associated with Neofibularia sp. The culturable
actinomycetes were Streptomyces spp., while rare actinomycetes were dominant when the metagenomic approach was
adopted. Several of these actinomycetes showed identities below 97% when matched to reference strains, indicating
possible novel species associated with the sponge Neofibularia.
- Full text:P020170105412706206665.pdf
