Citric acid production by Aspergillus niger using different substrates
- Author:
Chirova Travers Kudzai
;
Kumar Ajay
;
Panwar Ambika
- Publication Type:Journal Article
- Keywords:
Aspergillus niger;
carbon source;
citric acid (CA;
nitrogen source
- MeSH:
Citric Acid
- From:Malaysian Journal of Microbiology
2016;12(3):199-204
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Citric acid is a commercially important acid that has many applications in varying sectors of industries. It is
produced by various substrates through solid state or submerged fermentation. The capabilities of potato and rice as
substrates for citric acid production using Aspergillus niger were tested in this experiment under submerged
fermentation.
Methodology and results: Potato and rice extract media were prepared and inoculated with A. niger and titrations were
carried out to determine the amount of citric acid produced. It was shown that rice extract media proved more useful
than potato extract media as it produced the highest citric acid production. Rice extract media was supplemented with
varying concentrations of glucose and sucrose and 5% sucrose (w/v) proved to be the best as it produced the highest
amount of citric acid. The rice extract media with 5% sucrose (w/v) were supplemented with varying concentrations of
ammonium nitrate and ammonium sulphate and 0.25% ammonium nitrate proved more effective in citric acid production.
A low pH (1.9-2.3) was found during the maximum production of citric acid.
Conclusion, significance and impact of study: The results depict that potato and rice extract media can produce citric
acid, hence providing an alternate substrate for citric acid production.
- Full text:P020170105409130747037.pdf
