Isolation and characterization of an antifungal peptide from fruiting bodies of edible mushroom Lentinus squarrosulus Mont.
- Author:
Siwat Poompouang
;
Maneewan Suksomtip
- Publication Type:Journal Article
- Keywords:
Antifungal peptide;
antifungal activity;
Lentinus squarrosulus Mont.;
DEAE-cellulose;
Sephadex G-25
- MeSH:
Anti-Infective Agents
- From:Malaysian Journal of Microbiology
2016;12(1):43-49
- CountryMalaysia
- Language:English
-
Abstract:
Aims: To isolate and characterize an antimicrobial peptide from fruiting bodies of Lentinus squarrosulus Mont., the Thai
common edible mushroom.
Methodology and results: Solid ammonium sulfate at 40-80% (w/v) final concentration was utilized to precipitate the
proteins and further purified by ion exchange chromatography on DEAE-cellulose and gel filtration chromatography on
Sephadex G-25. The peptide was adsorbed on DEAE-cellulose and Sephadex G-25. It appeared as a single band with a
molecular mass of about 17 kDa (kilodalton) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Further
investigation of antimicrobial properties of purified peptide revealed that it has no activity against both Gram positive and
Gram negative bacteria. However, it exhibited strong antifungal activity against various species of fungal pathogen of
human. Among the high sensitive strains, Trichophyton mentagrophytes, T. rubrum, and Candida tropicalis are clinical
isolates. Moreover, the potency was found to be concentration dependent and comparable with Ketoconazole, the
commercial antifungal drug.
Conclusion, significance and impact study: In this work, the novel bioactive peptide from fruiting bodies of L.
squarrosulus Mont. has been isolated. It shows potent activity against various clinical isolates of fungal pathogen of
human. It may have potential for pharmaceutical application.
- Full text:P020160317494899966415.pdf
