Anti-biofilm activity of the marine bacterium Pseudoalteromonas ruthenica KLPp3 against Serratia marcescens and Vibrio alginolyticus
- Author:
Asmat Ahmad
;
Mohd Faiq Iqbal Sulieman
;
Gires Usup
- Publication Type:Journal Article
- Keywords:
Anti-microbial;
sub-MICs;
crude extract;
Anti-biofilm;
Pseudoalteromonas ruthenica
- MeSH:
Anti-Infective Agents
- From:Malaysian Journal of Microbiology
2016;12(1):30-34
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Pseudoalteromonas ruthenica KLPp3 is the marine Gram-negative strain isolated from the surface of mud crab at
Pulau Perhentian Malaysia. In this work, the anti-biofilm activity of P. ruthenica supernatant was examined on Serratia
marcescen and Vibrio alginolyticus.
Methodology and results: The crude extract of P. ruthenica KLPp3 was obtained using ethyl acetate. The subminimum
inhibitory concentration (MIC) of the crude extract was determined using the minimum inhibitory test. The subMIC
crude extract was tested against two of the S. marcescen virulence factors, which are the swarming ability and
production of prodigiosin. The crystal violet assay was used to test the anti-biofilm activity of the sub-MIC crude extract
against S. marcescen and V. alginolyticus. The productions of prodigiosin were reduced by 72%. The swarming area
was reduced by 56.06%. It inhibits 26.9% and 48.5% of biofilm production in S. marcescens and V. alginolyticus
respectively. The crude extract was heat stable.
Conclusion, significance and impact of study: Besides combating the S. marcescens virulence factor, P. ruthenica
KLPp3 crude extract in sub-MIC reduces the formation of biofilm of S. marcescens and V. alginolyticus, which may find
applications in biofilm inhibition and prevention.
- Full text:P020160317481111378413.pdf
