Multifunctional cellulolytic activities from Streptomyces osmaniensis for agricultural and enzyme industry
- Author:
Nuntavun Riddech
;
Nutthapon Saharm
;
Chanokporn Chaisawang
;
Paweenut Pongtongmee
;
Sutumporn Boonchern
;
Pornrapee Sarin
;
Thanaporn Phibunwatthanawong
- Publication Type:Journal Article
- Keywords:
β-glucosidase;
actinomycetes;
rice bra;
, rice blast pathogen
- MeSH:
Actinobacteria
- From:Malaysian Journal of Microbiology
2016;12(1):85-90
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The aims of this study were to screen for cellulose degradation activity from actinomycetes using agar plate
method, detect β-glucosidase activity, morphology and molecular taxonomy study.
Methodology and results: Preliminary screening for cellulose degrading Actinomycete was done on the carboxymethyl
cellulose agar (CMC agar) and detected by flooding with gram iodine. It was found that 190 isolates were cellulase
producing actinomycetes. Actinomycete isolate CDF2L1D13 showed maximum clear zone around the colony and the
highest hydrolysis capacity value was 3.93. β-glucosidase activity was examined by measuring the amount of paranitrophenol
(pNP) librated by Tako method. Study on comparison of the enzyme activity in CMC broth with alternative
broth was performed. The highest β-glucosidase activity was found on alternative production medium that supplemented
rice bran as a carbon source. β-glucosidase activity was 0.401 U/mL. The optimum pH of alternative production medium
for producing β-glucosidase was at pH value 7 and incubated at 30 °C. Isolate CDF2L1D13 was antagonistic
actinomycete against rice blast pathogen (Pyricularia oryzae). The character of this isolate was showed white color of
substrate mycelium, white color of aerial mycelium, gray spore and spiral spore chain. Actinomycete isolate CDF2L1D13
was phylogenetically similar to Streptomyces osmaniensis.
Conclusion, significance and impact of study: The result from this study indicated that Streptomyces osmaniensis
has the potential on β-glucosidase production and it is antagonistic actinomycete against Pyricularia oryzae.
- Full text:U020160317468072936020.pdf
