Isolation and identification of bacteria isolated from ruminant animal waste that able to degrade 2,2-dichloropropionic acid (2,2-DCP)
- Author:
Siti Nurul Fasehah Ismail
;
Roswanira Abdul Wahab
;
Fahrul Huyop
- Publication Type:Journal Article
- Keywords:
Cow dung;
halogenatedCow dung;
halogenated compound 2,2-dichloropropionic acid;
bioremediation Bacillus cereus
- MeSH:
Bacillus cereus
- From:Malaysian Journal of Microbiology
2016;12(2):155-163
- CountryMalaysia
- Language:English
-
Abstract:
Aims: A 2,2-dichloropropionic acid (2,2-DCP) naturally degrading bacterial species, strain SN1 was successfully
isolated from cow dung capable of utilizing the substance as the sole carbon source and energy.
Methodology and results: Strain SN1 was preferred over other strains (SN2, SN3 and SN4) following observations on
its rapid growth in 20 mM 2,2-DCP liquid minimal media. Since strain SN1 clearly exhibited tolerance towards 2,2-DCP,
its growth in various concentrations (10 mM, 20 mM, 30 mM and 40 mM) of the substance was evaluated. The study
found the bacteria grew particularly well in 20 mM 2,2-DCP with the highest chloride release of 39.5 µmole Cl-
/mL while
exhibiting a remarkably short doubling time of 3.85 h. In view of such notable characteristics, species identification via
Biolog GEN III system and 16S rRNA analysis was performed and established strain SN1 as Bacillus cereus.
Conclusion, significance and impact of study: Considering the rapid growth of B. cereus strain SN1 in such medium,
its employment as a bioremediation agent to treat 2,2-DCP contaminated soils may prove beneficial. Moreover, this is
the first reported case of a Bacillus sp. isolated from cow dung capable of utilizing 2,2-DCP. Therefore, further
assessment into its ability to degrade other types of haloalkanoic acids merit special consideration.
- Full text:P020160711349432345747.pdf
