Preliminary study of biofilm formation properties and antibiotic susceptibility pattern of MRSA and MSSA isolates obtained in Yogyakarta, Indonesia
- Author:
Titik Nuryastuti
;
Praseno Praseno
;
Muhammad Mustafa
- Publication Type:Journal Article
- Keywords:
MRSA;
biofilm;
icaA/D genes;
mecA gene;
antibiotic susceptibility
- MeSH:
Biofilms
- From:Malaysian Journal of Microbiology
2015;11(4):383-390
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Nowadays, Staphylococcus aureus, especially methicillin resistant Staphylococcus aureus (MRSA), emerged as
a major pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by these bacteria are often
difficult to treat because of the development of antibiotic resistance. Biofilm formation is an important factor in the
pathogenicity of staphylococcal infections and one of the reason of antibiotic treatment failure. In this study, the
relationship between biofilm formation properties, the presence of mecA, icaA/D genes and antimicrobial resistance
pattern were investigated in 10 methicillin sensitive Staphylococcus aureus (MSSA) and 10 MRSA clinical isolates.
Methodology and results: Staphylococcal strains were identified by conventional microbiological methods, while
determination of methicillin susceptibility was distinguished by the presence of mecA gene. To investigate biofilm
production, congo red agar and microtiter plate test were performed. PCR was done to detect the presence of icaA/D
genes, which responsible for biofilm production. Antibiotic sensitivity was carried out by agar diffusion method. The
majority of MRSA isolates (90%) were not able to form biofilm, only one isolate (10%) showed capability of weak biofilm
producer. Meanwhile, fully established biofilms were formed by all of MSSA isolates (100%). In addition, all MRSA and
almost MSSA isolates (90%) harboured both icaA/D genes in their chromosomes. Antibiotic resistance profile of MRSA
was more dominant than MSSA isolates.
Conclusion, significance, and impact of study: Biofilm production of staphylococci showed difference regulation with
regard to methicillin susceptibility. Antibiotic resistance profile was more dominant in MRSA, however biofilm production
was found mostly in MSSA isolates.
- Full text:P020151209437159858859.pdf
