Isolation, partial purification and characterization of thermophilic uricase from Pseudomonas otitidis strain SN4
- Author:
Nor Sahslin Irwan Shah Lee
;
Hamidreza Mansouri Khosravi
;
Norahim Ibrahim
;
Shafinaz Shahir
- Publication Type:Journal Article
- Keywords:
Uricase;
uric acid;
Pseudomonas otitidis;
hot springs
- MeSH:
Urate Oxidase
- From:Malaysian Journal of Microbiology
2015;11(4):352-357
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The present study aimed at isolating new source of uricase producers from Malaysian hot springs together with
partial purification and characterization of thermophilic uricase from novel strain.
Methodology and results: A bacteria strain, designated as SN4, was found to have the ability to degrade uric acid. 16S
rRNA analysis identified SN4 as Pseudomonas otitidis. Uricase was then extracted from SN4 and purification was
performed via ammonium sulphate precipitation. The effects of temperature, pH and metal ions on partially purified
uricase were evaluated. Results showed that 70% ammonium sulphate concentration gave the highest uricase activity at
4.18 U/mL compared to other concentrations. The molecular weight of the partially purified uricase was 33 kilodalton
(kDa). The optimum temperature for uricase was 45 °C and its activity was highest at pH 8.0. Calcium ions and copper
ions enhanced uricase activity while cobalt ions reduced uricase activity.
Conclusion, significance and impact of study: Isolation and investigation of uricase producers from new sources
such as thermophiles would increase availability and thermal stability of the uricase that could be used for significant
purposes such as in biochemical and clinical applications.
- Full text:P020151209384461880697.pdf
