Characterization of lignocellulolytic bacterial strains associated with decomposing wood residues in the Lagos lagoon, Nigeria
- Author:
Olanike Maria Buraimoh
;
Matthew Olusoji Ilori
;
Olukayode Oladipo Amund
- Publication Type:Journal Article
- Keywords:
Lignocellulose;
Streptomyces;
Bacillus;
Paenibacillus;
pollution
- MeSH:
Alcoholic Beverages;
Fermentation;
Phoeniceae
- From:Malaysian Journal of Microbiology
2015;11(3):273-283
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The presence of lignocelluloses, especially sawdust in the Lagos lagoon and the attendant ecological problems
warranted studies on their degradation. This study aimed to isolate and identify the indigenous bacterial strains capable
of utilizing lignocellulosic wastes under the prevalent tropical estuarine conditions.
Methodology and results: Nine bacterial species were obtained by elective culture from decomposing wood residues
in the lagoon. They were identified on the basis of morphology, biochemical characteristics and analysis of their 16S
rRNA gene sequences as Streptomyces, Bacillus and Paenibacillus species. They were cultured on various ligninrelated
lignocellulosic substrates over a period of 7 to 12 days. All the isolates showed moderate to very good growth on
sugarcane baggase. Streptomyces albogriseolus strain AOB and Paenibacillus sp. ROB showed good growth on grass
while on sawdust, only Streptomyces AOB, and Bacillus megaterium strain NOB showed good growth. High
performance liquid chromatographic analysis showed that the Streptomyces species completely utilized coniferyl
alcohol, B. megaterium strain NOB utilized 90-100% of all the lignin- related aromatic compounds. All the bacterial
species utilized less than 40% of sinapyl alcohol, Bacillus sp. OOB and Paenibacillus sp. strain ROB failed to utilize
vanillic acid.
Conclusion, significance and impact of study: The isolates degraded lignocellulosic wastes and lignin-related
compounds. The role of fungi in the breakdown of lignocellulose in the Lagos lagoon had been the subject of previous
research considerations whilst the role of bacteria spp was unreported. Autochthonous bacterial species may equally
play a role in the bio-rehabilitation of the sawdust-polluted water of the Lagos lagoon.
Keywords: Lignocellulose, Streptomyces, Bacillus, Paenibacillus, pollution
- Full text:P020151009339996137636.pdf
