Physicochemical screening of Candida lusitaniae P1 during synthesis of biosurfactant from coconut shell
- Author:
Simiat Olanike Jimoh
;
Nafisat Adesola Adefioye
;
Rashidat Ikeoluwa Bakare
;
Ramon Adegboyega Ibrahim
;
Abdul Adisa Ashorobi
- Publication Type:Journal Article
- Keywords:
Candida species;
biosurfactant;
drop collapse test;
emulsification index;
oil spreading technique
- MeSH:
Candida;
Fermentation
- From:Malaysian Journal of Microbiology
2015;11(3):306-312
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Fermentation and recovery are the major operating cost in biosurfactant production. Thus, the aim of this
research was to synthesize biosurfactant from agricultural residues using suitable fermentation and recovery techniques
in order to reduce the cost of production.
Methodology and results: Biosurfactant-producing yeast strains isolated from refined oil-contaminated soil samples
using yeast extract-diesel agar (YEDA) were subjected to physicochemical screening such as drop collapse test,
microplate analysis, oil spreading technique, emulsification index and thermostability. Based on the preliminary
screening result, Candida lusitaniae P1, C. parapsilosis P51, C. parapsilosis D3 and C. lusitaniae E1 were selected for
biosurfactant production using agricultural residue such as rice bran, wheat bran and coconut shell as substrate and
crude supernatant was analyzed by gas chromatography mass spectrometry. Candida lusitaniae P1 strain produced
98.96 g/L of biosurfactant from coconut shell but when subjected to mutagenesis the yield decreased to 52.24 g/L.
Conclusion, significance, and impact of study: The physicochemical properties of biosurfactant produced using
various carbon sources showed that coconut-shell is the best residue thus, variation in composition and concentration of
biosurfactant obtained implies that the quality and quantity of biosurfactant produced depends on the carbon source and
the genetic composition of the yeast isolate.
- Full text:P020151009338088636406.pdf
