Taxonomic characterization and isolation of antitrypanosomal compound from Streptomyces sp. FACC-A032 isolated from Malaysian forest soil
- Author:
Lili Sahira Husin1
;
Getha Krishnasamy
;
Muhd Syamil Azahar
;
Hema Thopla Govender
;
Norhayati Ismail
;
Muhd Haffiz Jauri
;
Siti Syarifah Mohd Mutalip
;
Mohd Ilham Adenan
- Publication Type:Journal Article
- Keywords:
Antitrypanosomal;
Streptomyces;
Malaysian soil actinobacteria;
bioassay-guided isolation;
Staurosporine
- MeSH:
Biological Assay;
Actinobacteria
- From:Malaysian Journal of Microbiology
2015;11(2):128-136
- CountryMalaysia
- Language:English
-
Abstract:
Aims: The present study is aimed at taxonomic characterization and isolation of active compound MS01 from
Streptomyces sp. FACC-A032 which exhibited strong antitrypanosomal activity (IC50 0.02 μg/mL).
Methodology and results: Isolate FACC-A032 was characterized based on its cultural, morphological, physiological
and genomic properties. Isolate FACC-A032 was tentatively identified as Streptomyces sp. Biochemical analysis of
diaminopimelic acid (DAP) isomer of whole-cell hydrolysates further confirmed the isolate FACC-A032 that contained
LL-DAP isomer as species belonging to the genus Streptomyces. The inoculum for submerged cultures of isolate FACCA032
was prepared from cultures on ISP2 agar. After eight days of growth at 28 2 °C and 200 rpm in fermentation
medium M3, fermentation broth was extracted with butanol and the crude extracts (solvent layer) were separated and
dried in vacuo. Further studies were carried out to isolate the active compound from the culture extracts of isolate FACCA032.
Using bioassay-guided isolation, crude extract was partitioned based on different polarity. After which, the
resulting elutes were tested for antitrypanosomal activity. The active fraction was analyzed with HPLC-DAD analysis.
Based on the analysis, major peak in the active fraction was collected using HPLC preparative. Active compound MS01
was isolated and structure elucidated using NMR spectroscopy.
Conclusion, significance and impact of study: Bioassay-guided isolation techniques used in this study had
discovered an active antitrypanosomal compound, staurosporine, from Streptomyces sp. FACC-A032. This is the first
discovery of staurosporine, a protein kinase inhibitor, from Malaysian soil actinobacteria Streptomyces sp. Therefore, the
study demonstrated the potential of Malaysian soil actinobacteria as antitrypanosomal therapeutic agent.
