Bioencapsulation of probiotic Lactococcus lactis subsp. lactis on Artemia franciscana nauplii: Effects of encapsulation media on Nauplii survival and probiotic recovery
- Author:
Jiun Yan Loh
;
Adeline Su Yien Ting
- Publication Type:Journal Article
- Keywords:
Artemia franciscana;
encapsulation media;
Lactococcus lactis subsp. lactis;
nauplii survival;
probiotic recovery
- MeSH:
Lactococcus lactis
- From:Malaysian Journal of Microbiology
2015;11(2):121-127
- CountryMalaysia
- Language:English
-
Abstract:
Aims: This study aimed to investigate the suitability and efficacy of various encapsulation media in bioencapsulating the
probiotic Lactococcus lactis subsp. lactis in Artemia franciscana nauplii. The impact of the encapsulation media on
nauplii survival and probiotic recovery was also determined.
Methodology and results: Various encapsulation media (sodium alginate, palm oil, starch, gum Arabic and gelatin)
were prepared by dissolving the respective media in artificial sea water. Each media was prepared in four different
concentrations: 0.25, 0.5, 1.0 and 2.0 g/L. To determine the suitability of encapsulation media on the survivability of A.
franciscana, survival rate (SR) of Artemia nauplii was determined after 8 hours post-encapsulation. Instar II stage
Artemia nauplii at 1 nauplii per mL was used for each replicate. The result revealed that A. franciscana reached 100 %
SR in the encapsulation media at ≤ 0.5 g/L. All media enabled > 23 % recovery of L. lactis subsp. lactis from
encapsulated A. franciscana, which is similar (p > 0.05) to the recovery of free-cells (non-encapsulated) of L. lactis
subsp. lactis. Noticeably in sodium alginate (E1) treatment, the total counts of L. lactis subsp. lactis in bioencapsulated
A. franciscana were the highest among others, accounting for 2.44 × 107 CFU/mL per A. franciscana tissue
homogenate.
Conclusion, significance and impact of study: Artemia nauplii bioencapsulated with L. lactis subsp. lactis using 0.5
g/L sodium alginate as encapsulation medium has the highest SR for nauplii and bioencapsulation efficiency,
respectively. This result provides a basic guideline for Artemia bioencapsulation in fish/shrimp larval culture.
- Full text:P020150716435080146785.pdf
