Antimicrobial resistance of Escherichia coli, Salmonella and enterococci isolated from surface of conventional broiler eggs, “Kampung” chicken eggs and carrying trays from wet markets in Selangor, Malaysia
- Author:
Aw Yoong Kit, Loong Mei-Jean
;
Yue Chii Xiang
;
Lee Kok Ming
;
Ng Sian Yee
;
Lee Sui Mae
- Publication Type:Journal Article
- Keywords:
Antimicrobial resistance;
chicken eggs;
Escherichia coli;
Salmonella;
enterococci
- MeSH:
Drug Resistance, Microbial;
Salmonella
- From:Malaysian Journal of Microbiology
2015;11(2):109-115
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Bacteria on chicken egg surfaces can be potential sources of food borne diseases. The aim of this study was to
determine the prevalence of E. coli, Salmonella and enterococci on the surface of conventional broiler eggs, “Kampung”
chicken eggs and carrying trays and to determine the antimicrobial resistant profile of these isolates.
Methodology and results: Conventional broiler eggs, “Kampung” chicken eggs and carrying trays were sampled
randomly from nine wet markets in Selangor, Malaysia. The surface of the eggs and carrying trays were swabbed and E.
coli, Salmonella and enterococci were isolated using selective agars. Antimicrobial susceptibility testing (AST) was
performed on the isolates against different antimicrobials via disk diffusion test. A large proportion of E. coli isolates
(>50% of isolates from conventional broiler eggs and “Kampung” chicken eggs) was resistant to chloramphenicol and
tetracycline whereas enterococci (>60% of isolates from conventional broiler eggs and “Kampung” chicken eggs)
isolates were resistant to tetracycline and erythromycin. Salmonella isolates were found to be susceptible to all of the
antimicrobials tested except for tetracycline. There was also presence of isolates showing multiple resistances in this
study. E. coli isolates (8.8%) from the surface of “Kampung” chicken eggs were resistant against 10 different
antimicrobials whereas 17.8% of the enterococci isolates from the surface of “Kampung” chicken eggs were resistant to
11 different antimicrobials.
Conclusion, significance and impact of study: The presence of multiple-antimicrobial resistant bacteria especially on
the surface of “Kampung” chicken eggs that are ready to be sold to consumers is a serious concern. However, further
study has to be conducted to determine the ultimate source of the bacterial contamination before specific food safety
measures can be introduced.
- Full text:P020150714610540807932.pdf
