Antimicrobial and enzymatic activities of endophytic bacteria isolated from Mentha spicata (MINT)
- Author:
Akinsanya Mushafau Adewale
;
Goh Joo Kheng
;
Lim Siew Ping
;
Ting Adeline Su Yien
- Publication Type:Journal Article
- Keywords:
Endophytic bacteria;
diversity;
antimicrobial;
enzymatic;
16S rDNA
- MeSH:
Anti-Infective Agents
- From:Malaysian Journal of Microbiology
2015;11(2):102-108
- CountryMalaysia
- Language:English
-
Abstract:
Aims: This study is to isolate and characterize endophytic bacteria for potential industrial enzymes and antimicrobial
activities against some human pathogens.
Methodology and results: Mentha spicata, a local medicinal plant, was used to source for endophytes. The roots,
stems and leaves of M. spicata were surface-sterilized to isolate the endophytic bacteria. The endophytic bacteria were
subsequently characterized based on their 16S rRNA sequences. The endophytic bacteria were screened for both
antimicrobial and enzymatic activities. We identified 15 isolates of 4 genus-Pseudomonas (7 species), Bacillus (3
species), Enterobacter (1 species) and Comamonas (1 species) with 97-100% similarity level. Isolates MSS-3
(Pseudomonas putida), MSR-4 (Pseudomonas pictorum), MSS-2 (Bacillus thuringiensis) and MSR-5 (Pseudomonas
straminea) showed prominent antimicrobial activities against the pathogens tested with zones of inhibition between 6.3
to 15.3 ± 0.6 mm. All species examined have positive cellulase activities except Comamonas guangdongensis and only
isolates of the Bacillus genus, Pseudomonas pictorum and P. argentinensis exhibited amylase activities.
Conclusion, significance and impact of study: Our findings revealed potential therapeutic uses of the bioactive
compounds of these bacteria endophytes against pathogens. Their enzymatic potential can also be of use in various
industries.
- Full text:P020150714602680492399.pdf
