Optimization of submerged culture for biomass and polysaccaharide of Pleurotus ostreatus BPPTCC 6017 using response surface methodology
- Author:
Priyo Wahyudi
;
Wibowo Mangunwardoyo
;
Wahono Sumaryono
;
Indrawati Gandjar
- Publication Type:Journal Article
- Keywords:
Oyster mushroom;
Pleurotus ostreatus BPPTCC 6017;
polysaccharide;
optimum condition;
central compositedesign;
submerged culture
- MeSH:
Fungal Polysaccharides
- From:Malaysian Journal of Microbiology
2015;11(1):27-39
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Polysaccharide of Pleurotus ostreatus is one of the fungal polysaccharide which has been widely studied,
produced by extracting the fruiting body. An alternative method for producing polysaccharide of P. ostreatus directly from
the mycelia instead of the fruiting body is through submerged culture. This study was aimed to determine the optimum
submerged culture conditions for producing biomass and intracellular polysaccharide of the oyster mushroom.
Methodology and results: P. ostreatus BPPTCC 6017 was collected from traditional mushroom farm in West Java,
Indonesia. Submerged fermentation was conducted in 1000 mL medium (2 L flask). Four variables were tested:
temperature, pH, agitation, and fermentation time, using central composite design of the response surface methodology.
Mycelial biomass produced, was extracted to obtain water-soluble and alkali-soluble polysaccharide. Experimental data
obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analysed by
appropriate statistical methods. The 3-D response surface plots derived from the mathematical models were applied to
determine the optimum conditions: temperature 27.89 °C, initial pH medium 5.49, agitation 124.08 rpm, and fermentation
time 11.44 days. The predicted results of the models were 33.75 g/L mycelia, 0.33 g/L water-soluble polysaccharide,
and 0.64 g/L alkali-soluble polysaccharide. Those results were then verified on the optimum conditions, and produced
32.00±1.25 g/L mycelia, 0.29±0.01 g/L water-soluble polysaccharide and 0.60±0.02 g/L alkali-soluble polysaccharide,
were close to the theoretical predictions.
Conclusion, significance and impact study: The present study was a first effort to assess and obtain the optimum
conditions for producing the biomass and polysaccharides of the strain P. ostreatus BPPTCC 6017 using submerged
fermentation
- Full text:P020160401450068326603.pdf
