Effects of Hibiscus sabdariffa Linn. Aqueous Extract on Oxidative Stress and T Lymphocyte Population in Spleen of Streptozotocin-induced Diabetic Rats
- Author:
Nor Malia Abd Warif
;
Asyraf Akmal Ayob
;
Wan Marahaini Wan Razali
;
Siti Balkis Budin
;
Satirah Zainalabidin
;
Jamaludin Mohamed
- Publication Type:Journal Article
- Keywords:
Diabetes, H. sabdariffa, spleen, oxidative stress, T lymphocytes
- From:Malaysian Journal of Health Sciences
2014;12(1):15-21
- CountryMalaysia
- Language:English
-
Abstract:
Disturbances in immune system contribute to chronic infection among diabetic patients. Hibiscus sabdariffa L. (roselle)
fruit extract has been scientifi cally proven to possess antioxidant, antidiabetic and antiinfl ammatory properties. The
aim of this study was to investigate the effects of H. sabdariffa fruit extract against oxidative stress parameter and
T lymphocyte population in spleen of streptozotocin (STZ) induced diabetic rats. Male Sprague-Dawley rats were injected
with 45 mg/kg STZ to induce diabetic condition and further treated with 100 mg/kg H. sabdariffa fruit aquoeus extract
daily for 28 days. Spleen was harvested to determine the oxidative stress indicators and quantifi cation of T lymphocytes.
The results showed a signifi cant decreased in the number of spleen cells and spleen weight in the diabetic rats compared
with control rats. However, there were no signifi cant changes in the level of malondialdehyde (MDA), protein carbonyl and
superoxide dismutase (SOD) activity the percentage of spleen CD3+ CD4+ and CD3+ CD8+ T lymphocytes amongst groups
of study. In addition, histology observation showed no pathological alteration in spleen histology of diabetic rats. The
fi ndings suggested that aqueous extract of H. Sabdariffa fruit supplementation has no effect on the oxidative stress and
the percentage of CD3+ CD4+ and CD3+ CD8+ T lymphocytes in spleen of diabetic rats
- Full text:W020150511315238996846.pdf
