Manufacture of MAC-ELISA applied in diagnosis of measles IgG antibody
- Author:
Nguyen Hanh Phuc
- Publication Type:Journal Article
- Keywords:
Measles
- MeSH:
Measles;
Diagnosis;
Immunoglobulin G
- From:Journal of Practical Medicine
2005;503(2):38-39
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Study serum of 443 health adults over 20 years old, negativeness with HIV and HBV and serum of 408 health childrens from 2 to 10 years old in Ha noi, Hai Phong and Thanh Hoa. Measles antigen were refined from Vero cell causing infection of Edmonton strain by Gradi extra centrifugal method with Sucrose density 20-60%. HA value of 1/11,024 showed high antigen specificity. 6 bunchs of protein were correllated with specific polypeptid strings of measles virus. After that, technical process and MAC ELISA-IgG were completed. Results: rate of measles immune response in adults was 100%, in which IgG value was high in 24%, moderate in 73.1% and low in 2.8%. Rate of serum IgG value in children was different to adults: positive serum 88%, negative 8.5%. Negative rate showed that the vaccine boosters were necessary
