Production of citric acid by Aspergillus niger immobilized in Detarium microcarpum matrix
- Author:
Kareem Sarafadeen Olateju
;
Oladipupo Idayat Omowumi
;
Omemu Adebukunola Mobolaji
;
Babajide Joan Modupe
- Publication Type:Journal Article
- Keywords:
Citric acid;
Detarium microcarpum;
Aspergillus niger;
cassava whey
- From:Malaysian Journal of Microbiology
2013;9(2):161-165
- CountryMalaysia
- Language:English
-
Abstract:
Aims: This study investigated the potential use of Detarium microcarpum as a support matrix for the immobilization of fungal cell and its subsequent use in the production of citric acid.
Methodology and results: Conidia of Aspergillus niger were immobilized on Detarium microcarpum matrix crosslinked
with glutaraldehyde (2.5% v/v). Effects of various immobilized conditions on citric acid yield were determined. Citric acid production was optimum at 9% gel concentration, 5.0 mm bead size and 400 mg spore load. Higher citric acid yield (82g/L) was obtained by immobilized cells while free cells gave (61 g/L). Immobilized gels were stable for 15 days in the
fermentation medium and retained about 75% of initial yield after five repeated cycles.
Conclusion, significance and impact of study: This study therefore shows that D. microcarpum is a novel matrix for
cell immobilization and will enhance production of citric acid in batch system.
- Full text:P020160926541722382259.pdf