Waste cooking oil as substrate for biosynthesis of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate): Turning waste into a value-added product
- Author:
Hanisah Kamilah
;
Takeharu Tsuge
;
Tajul Aris Yang
;
Kumar Sudesh
- Publication Type:Journal Article
- Keywords:
Waste cooking oil;
Poly(3-hydroxybutyrate);
Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Cupriavidus necator H16, Cupriavidus necator PHBˉ4
- From:Malaysian Journal of Microbiology
2013;9(1):51-59
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Improper disposal of domestic wastes, such as waste cooking oil (WCO), contributes to the deterioration of the
environment and may lead to health problems. In this study, we evaluated the potential of plant-based WCO as a carbon source for the commercial biosynthesis of the bio-plastics, poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). The consumption of WCO for this purpose would mitigate their pollution of the environment at the same time.
Methodology and Results: WCO collected from several cafeterias in USM was tested as the carbon source for
polyhydroxyalkanoates (PHA) production. A selection of suitable nitrogen source was first conducted in order to obtain an acceptable number of dry cell weight (DCW) and PHA content. Urea was found to be a suitable nitrogen source for the two bacterial strains used in our study, Cupriavidus necator H16 and its transformed mutant, C. necator PHB¯4 harboring the PHA synthase gene of Aeromonas caviae (PHB¯4/pBBREE32d13). With WCO as the sole carbon source, C. necator H16 yielded a relatively good dry cell weight (DCW=25.4 g/L), with 71 wt% poly(3-hydroxybutyrate) P(3HB) content. In comparison, the DCW obtained with fresh cooking oil (FCO) was 24.8 g/L. The production of poly(3
hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] from WCO by the transformant C. necator PHB¯4 was
comparable, yielding a DCW of 22.3 g/L and P(3HB-co-3HHx) content of 85 wt%. Lipase activities for both bacterial
strains reached a maximum after 72 h of cultivation when time profile was conducted.
Conclusion, significance and impact of study: The use of WCO as a carbon source in the biosynthesis of the bioplastic, PHA, turns a polluting domestic waste into a value-added biodegradable product. This renewable source
material can thus be exploited for the low cost production of PHA.
- Full text:P020160926361115048126.pdf
