Production of some biologically active secondary metabolites from marine-derived fungus Varicosporina ramulosa
- Author:
Atalla, M. Mabrouk.
;
Zeinab, H. Kheiralla.
;
Eman, R. Hamed.
;
Amani, A. Youssry
;
Abeer, A. Abd El Aty
- Publication Type:Journal Article
- Keywords:
Marine fungi, Cytotoxicity, Pharmaceuticals, Secondary metabolites, anticancer compounds, Varicosporina ramulosa.
- From:Malaysian Journal of Microbiology
2008;4(1):14-24
- CountryMalaysia
- Language:English
-
Abstract:
In a screening of fungal isolates associated with marine algae collected from Abou-keer, Alexanderia during the four seasons of 2004, to obtain new biologically active compounds. Varicosporina ramulosa isolate was identified and selected as a producer of 13 compounds. Out of 13 pure compounds produced, compounds 3 and 10 were considered as antibacterial and antifungal compounds, respectively as they were active against gram positive, gram negative bacteria and a fungus.
Optimization of conditions (fermentation media, incubation period, temperature, initial pH, aeration levels) which activate compounds 3 and 10 production were studied. Also the spectral properties (UV, MS, GC/MS, IR and 1H-NMR) of the purified compounds were determined. Compound 3 suggested to be dibutyl phthalate and compound 10 may be ergosterol or one of its isomers. Biological evaluation of the two compounds towards 6 different types of tumor cell lines showed weak effect of compound 3 at different concentrations on the viable cell count of the different tumor cell lines. While compound 10 showed
different activities against the viable cell count of the 6 different tumor cell lines. It kills 50% of the viable infected liver and lung cells at concentrations equal to 99.7 μg/mL, 74.9μg/mL, respectively. Compound 10 can be recommended as new anticancer compounds.
- Full text:P020160923351279109272.pdf
