The Effect of Urocortin 1 on Motility in Isolated, Vascularly Perfused Rat Colon.
10.4166/kjg.2015.65.5.283
- Author:
Il Young YOU
1
;
Seungho LEE
;
Ki Bae KIM
;
Hee Seung LEE
;
Jong Soon JANG
;
Myeongho YEON
;
Joung Ho HAN
;
Soon Man YOON
;
Hee Bok CHAE
;
Seon Mee PARK
;
Sei Jin YOUN
Author Information
1. Department of Internal Medicine, Chungbuk National University College of Medicine, Cheongju, Korea. sjyoun@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Urocortins;
Corticotropin-releasing factor;
Rats;
Cholinergic receptors;
Muscarinic receptors
- MeSH:
Animals;
Colon/*drug effects/physiology;
Injections, Intravenous;
Male;
Muscle Contraction/drug effects;
Neurotransmitter Agents/pharmacology;
Rats;
Rats, Sprague-Dawley;
Receptors, Cholinergic/chemistry/metabolism;
Urocortins/isolation & purification/*pharmacology
- From:The Korean Journal of Gastroenterology
2015;65(5):283-290
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Urocortin 1, a corticotropin-releasing factor related peptide, increases colonic motility under stressful conditions. We investigated the effect of urocortin 1 on colonic motility using an experimental model with isolated rat colon in which the blood flow and intestinal nerves were preserved. Furthermore, we assessed whether this effect was mediated by adrenergic or cholinergic nerves. METHODS: Colonic motility was measured in the proximal and distal parts of resected rat colon. The colon resected from the peritoneum was stabilized, and then urocortin 1 (13.8, 138, 277, and 1,388 pM) was administered via a blood vessel. Motility index was measured in the last 5 min of the 15 min administration of urocortin 1 and expressed as percentage change from baseline. Subsequently, the change in motility was measured by perfusing urocortin 1 in colons pretreated with phentolamine, propranolol, hexamethonium, atropine, or tetrodotoxin. RESULTS: At concentrations of 13.8, 138, 277, and 1,388 pM, urocortin 1 increased the motility of proximal colon (20.4+/-7.2%, 48.4+/-20.9%, 67.0+/-25.8%, and 64.2+/-20.9%, respectively) and the motility of distal colon (3.3+/-3.3%, 7.8+/-7.8%, 71.1+/-28.6%, and 87.4+/-32.5%, respectively). The motility induced by urocortin 1 was significantly decreased by atropine to 2.4+/-2.4% in proximal colon and 3.4+/-3.4% in distal colon (p<0.05). However, tetrodotoxin, propranolol, phentolamine, and hexamethonium did not inhibit motility. CONCLUSIONS: Urocortin 1 increased colonic motility and it is considered that this effect was directly mediated by local muscarinic cholinergic receptors.
