Association between Oxidative DNA Damage, Fruits and Vegetables Intake with Breast Cancer: A Preliminary Study in Klang Valley
- Author:
Suzana Shahar
;
Kim Tiu Teng
;
Nor Fadilah Rajab
;
Fatimah Arshad
- Publication Type:Journal Article
- Keywords:
DNA oxidative damage, Breast cancer, Alkaline Comet Assay, Fruits and vegetables
- From:Malaysian Journal of Health Sciences
2008;6(1):61-77
- CountryMalaysia
- Language:English
-
Abstract:
A preliminary study was conducted to determine the level of oxidative DNA damage, fruits and vegetables intake among 50 breast cancer patients (cases)
as compared to 50 healthy women (controls) with no known medical history of breast cancer in Klang Valley. Both groups were matched for age and ethnicity.
Data on socio-demographic, health status and medical history, fruits and vegetables intake, and supplements intake were obtained through an interviewbased
questionnaire. Anthropometry measurements included weight, height, and waist and hip circumference were also carried out on subjects. A total of 3mL fasting venous blood was drawn to assess lymphocytes oxidative DNA
damage using Alkaline Comet Assay. Results indicated that the mean intake of fruits and vegetables was lower in cases (4.09 ± 1.17 servings/d) than controls
(4.77 ± 0.90 servings/d)(p < 0.05) The intake of fruits and vegetables from family groups of solanaceae, myrtaceae, caricaceae, apiaceae, brinjal, rutaceae,
broccoli, orange, carrot, watermelon were 0.5 - 1 servings/week significantly higher among controls as compared to cases (p < 0.05 for all parameters).
However, the intake of fruits from rosaceae family and apple was higher among controls than cases (p < 0.05). The estimated intake of β-carotene, carotenoids,
vitamin A, vitamin C (p < 0.001), α-carotene and lycopene (p < 0.05) from fruits and vegetables were higher among controls than cases. Mean DNA damage
level of cases (4.55 ± 1.78 % DNA in tail, %TD; 0.35 ± 0.21 tail moment, TM) were 3.5 and 3.9 times higher than the value of controls (1.3 ± 0.70% TD; 0.09
± 0.09 TM) (p < 0.001) and the damage increased with higher values of waist hip ratio (% TD, r = 0.396, p < 0.05; TM, r = 0.349, p < 0.05) and waist
circumference (% TD, r = 0.334, p < 0.05; TM, r = 0.360, p < 0.05). There was an inverse relationship between oxidative DNA damage with intake of total
fruits and vegetables, cauliflowers and water convolvulus and also consumption from rutaceae and solanaceae families. Similar trend was noted for estimated intake of vitamin A, carotenoids, vitamin C, β-carotene and lycopene. In conclusion, the intake of fruits and vegetables of five servings/d and the
consumption of specific families and types of fruits and vegetables might protect against oxidative DNA damage and further reduce breast cancer risk.
- Full text:W020150619352721333836.pdf
