Immobilization of Mucor racemosus NRRL 3631 lipase with different polymer carriers produced by radiation polymerization
- Author:
Mostafa, H. and El-Hadi, A. A.
- Publication Type:Journal Article
- Keywords:
lipase, Mucor racemosus, polymer carriers, radiation polymerization
- From:Malaysian Journal of Microbiology
2010;6(2):149-155
- CountryMalaysia
- Language:English
-
Abstract:
Lipase was partially purified from the culture supernatant of Mucor racemosus NRRL 3631. In an attempt to increase the
enzyme stability, the enzyme was immobilized on poly (vinyl alcohol) PVA, radiation cross liked poly (vinyl alcohol/ vinyl
pyrrolidone) PVA / PVP and poly (vinyl alcohol/ hydroxyethylmethacrylate) PVA/ HEMA hydrogels. The maximum
immobilization yield (31.74 %) was obtained using PVA/ HEMA copolymer. The effect of the immobilization parameters
on the enzyme such as the hydrogel composition, irradiation dose and the immobilization technique was performed. An
optimum radiation dose of 15 kGy and hydrogel composition of 10 % PVA/ HEMA (9.6: 0.4 v/v) increased the
immobilization yield to 60.3 %. Diffusion phenomena can be markedly increased the enzyme immobilization on the
surface of the hydrogel. In this case the retained activity was approximately 81.5 % of that of the free enzyme. The
profiles of immobilized enzyme activities at various pH values (4-9) and temperatures (30-80 °C) showed an overall
higher stability for the immobilized enzyme than that for the free one. The half life values of the immobilized and free
enzymes at 60 °C were 3.3 h and 1.73 h, respectively. The immobilized enzyme retained 69.2 % of its initial activity after
three cycles.
- Full text:P020160923479945669742.pdf
