Comparative Study of in vivo Gastrointestinal Absorption of Mustard Oil Emulsions Prepared with Different Types of Medium Chain Fatty Acids
- Author:
Susmita Roy
;
Sohini Mukherjee
;
Avery Sengupta
;
Mahua Ghosh
- Publication Type:Journal Article
- Keywords:
Intestinal absorption, medium chain fatty acids, oil-in-water emulsion
- From:
Malaysian Journal of Nutrition
2017;23(1):107-115
- CountryMalaysia
- Language:English
-
Abstract:
Absorption of dietary fats is generally in the form of emulsions. The present
study assessed the preparation and gastrointestinal absorption efficiency of three emulsions
of mustard oil containing three types of medium chain fatty acids (MCFAs) in a rat model.
Methods: Caprylic acid (C8:0), capric acid (C10:0) and lauric acid (C12:0) were chosen as the
MCFAs. Mustard oil emulsions were formulated using each of the MCFAs and lecithin as
an emulsifier. The characteristics of the formulations including optical microscopy, particle
size, zeta potential analysis and viscosity studies were assessed. Thereafter the intestinal
digestion patterns of the three MCFA rich mustard oil emulsions were compared using a
single pass perfusion test. Results: The particle size of the emulsions varied between 212.70
nm and 312.70 nm. Physical characterisation such as a zeta potential study confirmed that
all emulsions were thermodynamically stable. The absorption study was monitored at
30 min intervals of up to 2 h The absorption of C8:0 emulsion was found to be maximum
(27.78%) followed by C10:0 emulsion (24.81%) and C12:0 emulsion (22.50%). The differences
in absorption efficiency of the emulsions could be attributed to the smaller chain length of
C8:0 which was more rapidly absorbed by the intestine. Conclusion: In vivo gastrointestinal
absorption of MCFA rich mustard oil emulsions was compared; caprylic acid-rich mustard
oil showed the highest absorption rate in comparison to the other two emulsions. Further
in vivo studies are required to establish the mechanism of absorption of structured lipids
containing MCFAs.
- Full text:P020170515382325409204.pdf
