Effect of perivitelline fluid from horseshoe crab on the expression of COL1A1 in dental pulp stem cells
- Author:
Amanina Fatinah binti Kamarudin
;
Najian binti Ibrahim
;
Ahmad Aizat Abdul Aziz
;
Thirumulu Ponnuraj Kannan
- Publication Type:Journal Article
- Keywords:
cell cycle;
COL1A1;
dental pulp stem cells;
gene expression;
horseshoe crab;
perivitelline fluid
- MeSH:
Dental Pulp;
Horseshoe Crabs;
Stem Cells
- From:Archives of Orofacial Sciences
2016;11(2):26-30
- CountryMalaysia
- Language:English
-
Abstract:
Perivitelline fluid, extracted from the fertilized eggs of horseshoe crabs, has been reported to play a
vital role in supporting embryogenesis as well as cell proliferation. The present study aims to evaluate the effect
of PVF on the expression of COL1A1 in human dental pulp stem cells (DPSCs). The cells were grouped into two;
untreated (control) and treated with a single dose of PVF (0.019 mg/ml). Gene expression was quantified for
COL1A1 on day 1, 3 and 7 using reverse transcriptase PCR. The expression of COL1A1 on day 3 of treated
group with PVF was the highest though there was a decline of COL1A1 expression on day 7. Mann Whitney test
was utilized to determine the significance of COL1A1 expression between treated and untreated groups.
Significant difference in the expression of COL1A1 was observed between the treated and untreated groups on
day 3 though there was no significance in the expression on day 7. The present study indicates that PVF may
have the potential to increase cell proliferation in human DPSCs.
- Full text:P020170109450975705084.pdf
