Identification of Rotavirus Strains Causing Diarrhoea in Children under Five Years of Age in Yogyakarta, Indonesia
- Author:
Hera Nirwati
;
Mohamad Saifudin Hakim
;
Abu Tholib Aman
- Publication Type:Journal Article
- Keywords:
rotavirus, diarrhoea, G-type, P-type, children
- From:Malaysian Journal of Medical Sciences
2017;24(2):68-77
- CountryMalaysia
- Language:English
-
Abstract:
Background: Rotavirus is an important cause of severe diarrhoea in children. The aims of
this study were to identify the rotavirus strains that cause diarrhoea in children in Yogyakarta and
to determine the association between rotavirus positivity and its clinical manifestations.
Methods: Clinical data and stool samples were collected from children hospitalised at
Kodya Yogyakarta Hospital, Indonesia. Rotavirus was detected in stool samples using an enzyme
immunoassay (EIA), which was followed by genotyping using reverse transcriptase polymerase
chain reaction (RT-PCR). Electropherotyping was performed for the rotavirus-positive samples.
Results: In total, 104 cases were included in the study, 57 (54.8%) of which were
rotavirus-positive. Based on a multiple logistic regression analysis, age group, vomiting and stool
mucous were associated with rotavirus positivity. Most of the 56 samples subjected to genotyping
were classified as G1 (80.36%) and P[8] (69.64%) genotypes. The genotype combination
G1P[8] was identified as the most prevalent strain (66.07%). Of the 19 samples subjected to
electropherotyping, 17 G1 isolates and 1 G3 isolate had long patterns, and 1 G1 isolate had a short
pattern.
Conclusion: G1P[8] was the most dominant strain of rotavirus causing diarrhoea in
children in Yogyakarta. Age group, vomiting and stool mucous were associated with rotavirus
positivity.
- Full text:P020170508569593531864.pdf
