Attachment, Proliferation, and Morphological Properties of Human Dermal Fibroblasts
- Author:
Fauzi Mh Busra
;
Yogeswaran Lokanathan
;
Aminuddin Saim
;
Ruszymah Bt Hj Idrus
;
Shiplu Roy Chowdhury
- Publication Type:Journal Article
- Keywords:
collagen type I, hydrogel, proliferation, fibroblasts, tissue engineering
- From:Malaysian Journal of Medical Sciences
2017;24(2):33-43
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered
substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine
tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological
features of human dermal fibroblasts (HDF) on the scaffolds.
Methods: This study was conducted between the years 2014 to 2016 at the Tissue
Engineering Centre, UKM Medical Centre. OTC-I was extracted from ovine tendon, and fabricated
into 3D scaffolds in the form of sponge, hydrogel and film. A polystyrene surface coated with
OTC-I was used as the 2D culture condition. Genipin was used to crosslink the OTC-I. A noncoated
polystyrene surface was used as a control. The mechanical strength of OTC-I scaffolds
was evaluated. Attachment, proliferation and morphological features of HDF were assessed and
compared between conditions.
Results: The mechanical strength of OTC-I sponge was significantly higher than that of
the other scaffolds. OTC-I scaffolds and the coated surface significantly enhanced HDF attachment
and proliferation compared to the control, but no differences were observed between the scaffolds
and coated surface. In contrast, the morphological features of HDF including spreading, filopodia,
lamellipodia and actin cytoskeletal formation differed between conditions.
Conclusion: OTC-I can be moulded into various scaffolds that are biocompatible and thus
could be suitable as scaffolds for developing tissue substitutes for clinical applications and in
vitro tissue models. However, further study is required to determine the effect of morphological
properties on the functional and molecular properties of HDF.
- Full text:P020170508546881969189.pdf
