In Vitro Antifungal Activities against Moulds Isolated from Dermatological Specimens
- Author:
Tzar Mohd Nizam
;
Rabiatul Adawiyah AG. Binting
;
Shafika Mohd Saari
;
Thivyananthini Vijaya Kumar
;
Marianayati Muhammad
;
Hartini Satim
;
Hamidah Yusoff
;
Jacinta Santhanam
- Publication Type:Journal Article
- Keywords:
antifungal;
dermatology;
mold;
amphotericin B;
azoles;
terbinafine
- From:Malaysian Journal of Medical Sciences
2016;23(3):32-39
- CountryMalaysia
- Language:English
-
Abstract:
Background: This study aimed to determine the minimum inhibitory concentrations (MICs) of various antifungal agents against moulds isolated from dermatological specimens. Methods: We identified 29 moulds from dermatological specimens between October 2012 and March 2013 by conventional methods. We performed antifungal susceptibility testing on six antifungal agents, amphotericin B, clotrimazole, itraconazole, ketoconazole, miconazole and terbinafine, according to the Clinical and Laboratory Standards Institute guidelines contained in the M38-A2 document. Results: Most antifungal agents were active against the dermatophytes, except for terbinafine against Trichophyton rubrum (geometric mean MIC, MICGM 3.17 µg/mL). The dematiaceous moulds were relatively susceptible to amphotericin B and azoles (MICGM 0.17-0.34 µg/mL), but not to terbinafine (MICGM 3.62 µg/mL). Septate hyaline moulds showed variable results between the relatively more susceptible Aspergillus spp. (MICGM 0.25-4 µg/mL) and the more resistant Fusarium spp. (MICGM 5.66-32 µg/mL). The zygomycetes were susceptible to amphotericin B (MICGM 0.5 µg/mL) and clotrimazole (MICGM 0.08 µg/mL), but not to other azoles (MICGM 2.52-4 µg/mL). Conclusion: Amphotericin B and clotrimazole were the most effective antifungal agents against all moulds excepting Fusarium spp., while terbinafine was useful against dermatophytes (except T. rubrum) and Aspergillus spp. However, a larger study is required to draw more solid conclusions.
- Full text:P020160531379670682585.pdf
